# What drugs do laboratories test for?

**Citation:** 49 CFR 40.82  
**Type / status:** regulation / current  
**Agency:** Pipeline and Hazardous Materials Safety Administration  
**Effective:** 2026-08-18  
**Published:** Not stated

As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs.

(a) Marijuana metabolites.

(b) Cocaine metabolites.

(c) Amphetamines.

(d) Opioids.

(e) Phencyclidine (PCP).

[82 FR 52244, Nov. 13, 2017. Re

## Document text

As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs.

(a) Marijuana metabolites.

(b) Cocaine metabolites.

(c) Amphetamines.

(d) Opioids.

(e) Phencyclidine (PCP).

[82 FR 52244, Nov. 13, 2017. Redesignated at 88 FR 27643, May 2, 2023]

## Provenance

- Official: Yes
- Source: <https://www.ecfr.gov/on/2026-08-18/title-49/section-40.82>
- Source ID: `ecfr`
- SHA-256: `15f091bac2615ec908628784cacac03ed56f5f6f620e705237ab126cbf617cfd`
- Retrieved: 2026-08-20T05:54:21.729Z
- Exported: 2026-08-23T15:07:44.805Z
- Document slug: `49-cfr-40-82`

### Source metadata

```json
{
  "cfrTitle": 49,
  "part": "40",
  "section": "40.82",
  "subpart": "Subpart F—Drug Testing Laboratories",
  "corpusVersion": "2026-08-18",
  "jurisdiction": "US"
}
```