# What does the second laboratory do with the split specimen when it is tested to reconfirm an adulterated test result?

- **operation:** document
- **citation:** 49 CFR 40.179
- **title:** What does the second laboratory do with the split specimen when it is tested to reconfirm an adulterated test result?
- **source type:** regulation
- **agency:** Pipeline and Hazardous Materials Safety Administration
- **status:** current
- **official:** true
- **published on:** Not available
- **effective on:** 2026-08-18
- **summary:** (a) As the laboratory testing the split specimen, you must test the split specimen for the adulterant detected in the primary specimen, using the confirmatory test for the adulterant and using criteria in § 40.89 or § 40.93, as applicable and confirmatory cutoff levels required by the HHS Mandatory 
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- **source url:** https://www.ecfr.gov/on/2026-08-18/title-49/section-40.179
**body:**

(a) As the laboratory testing the split specimen, you must test the split specimen for the adulterant detected in the primary specimen, using the confirmatory test for the adulterant and using criteria in § 40.89 or § 40.93, as applicable and confirmatory cutoff levels required by the HHS Mandatory Guidelines.

(b) In addition, if the test fails to reconfirm the adulterant result reported in the primary specimen, you may send the specimen or an aliquot of it for testing at another HHS-certified laboratory that has the capability to conduct another reconfirmation test.

[73 FR 35973, June 25, 2008, as amended at 88 FR 27646, May 2, 2023]
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- **body characters:** 644