Subpart A—Administrative Provisions4Subpart B—Employer Responsibilities11Subpart C—Specimen Collection Personnel4Subpart D—Collection Sites, Forms, Equipment and Supplies Used in DOT Urine and Oral Fluid Collections10Subpart E—Specimen Collections11Subpart F—Drug Testing Laboratories18Subpart G—Medical Review Officers and the Verification Process26Subpart H—Split Specimen Tests9Subpart I—Problems in Drug Tests12Subpart J—Alcohol Testing Personnel3Subpart K—Testing Sites, Forms, Equipment and Supplies Used in Alcohol Testing8Subpart L—Alcohol Screening Tests4Subpart M—Alcohol Confirmation Tests3Subpart N—Problems in Alcohol Testing9Subpart O—Substance Abuse Professionals and the Return-to-Duty Process16Subpart P—Confidentiality and Release of Information7Subpart Q—Roles and Responsibilities of Service Agents8Subpart R—Public Interest Exclusions27
Regulationcurrent
49 CFR 40.82
What drugs do laboratories test for?
Current as of 2026-08-18 Government source
eCFR editorial compilationAuthoritative but unofficial
As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test “DOT specimens” for any other drugs.#
RegulusPipeline research